5x primescript rt master mix (TaKaRa)
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5x primescript rt master mix
5x Primescript Rt Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+master+mix/PrimeScript+RT+Master+Mix/pm42894224-77-12-17
Average 99 stars, based on 27259 article reviews
5x Primescript Rt Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+rt+master+mix/PrimeScript+RT+Master+Mix/pm42894224-77-12-17
Average 99 stars, based on 27259 article reviews
5x primescript rt master mix - by Bioz Stars,
2026-11
99/100 stars
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Reverse Transcription:Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease Article Snippet: Total RNA was extracted from tissues or cells using TRIzol reagent (Invitrogen, USA). .. Reverse transcription was performed using Article Title: Chlorella sorokiniana attenuates deoxynivalenol-exacerbated allergic dermatitis and type 2 immune responses in mice Article Snippet: Total RNA was extracted from the homogenized tissues using the NucleoSpin RNA kit (TaKaRa Bio Inc., Shiga, Japan) according to the manufacturer’s instructions. .. Subsequently, 120 ng of total RNA was reverse transcribed into complementary DNA using Article Title: Impact of Fish Meal Replacement with Meat Meal on the Growth and Fillet Nutritional Quality of Chum Salmon ( Oncorhynchus keta ). Article Snippet: Total RNA purity and concentration were measured using a BioTek Synergy HTX Multimode Microplate Reader (Agilent Technologies, Santa Clara, CA, USA). .. cDNA was synthesized for quantitative reverse transcription polymerase chain reaction (qRT-PCR) using the Article Title: Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD + Depletion and Osteogenic Decline in Skeletal Aging Article Snippet: .. Total RNA was extracted from cells using TRNzol Universal (TIANGEN, DP424) and reverse‐transcribed into cDNA using the Article Title: Integrative multi-omics analysis defines a macrophage-associated phospholipid metabolism signature linked to immune remodeling and prognosis in lung adenocarcinoma. Article Snippet: .. Equal amounts of RNA were reverse transcribed into cDNA using Article Title: GSTM5 suppresses ovarian cancer progression in association with RASSF2A promoter hypomethylation and ferroptosis. Article Snippet: Objective: Glutathione S-transferase Mu 5 (GSTM5) is a detoxification-related enzyme whose function in ovarian cancer (OC) remains incompletely understood.. This study investigated the association of GSTM5 with OC progression and examined whether mitochondrial dysfunction, ferroptosis-related injury, and Ras association domain family member 2 isoform A (RASSF2A) promoter methylation contribute to its effects.. Methods: Gain‐ and loss‐of‐function experiments were performed in OC cells. Synthesized:Article Title: Impact of Fish Meal Replacement with Meat Meal on the Growth and Fillet Nutritional Quality of Chum Salmon ( Oncorhynchus keta ). Article Snippet: Total RNA purity and concentration were measured using a BioTek Synergy HTX Multimode Microplate Reader (Agilent Technologies, Santa Clara, CA, USA). .. cDNA was synthesized for quantitative reverse transcription polymerase chain reaction (qRT-PCR) using the Article Title: Genome-Wide Identification and Characterization of the UMAMIT Gene Family in Brassica napus : Evolution, Expression, and Potential Association with Oil Content. Article Snippet: .. First-strand cDNA was synthesized using Polymerase Chain Reaction:Article Title: Impact of Fish Meal Replacement with Meat Meal on the Growth and Fillet Nutritional Quality of Chum Salmon ( Oncorhynchus keta ). Article Snippet: Total RNA purity and concentration were measured using a BioTek Synergy HTX Multimode Microplate Reader (Agilent Technologies, Santa Clara, CA, USA). .. cDNA was synthesized for quantitative reverse transcription polymerase chain reaction (qRT-PCR) using the other:Article Title: Akkermansia muciniphila attenuates macrophage efferocytosis to enhance anti-PD-1 efficacy in microsatellite-stable colorectal cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse IL-6 ELISA Kit Jianglai Biotechnology Co., Ltd. Cat#JL20268 Mouse TNF-α ELISA Kit Jianglai Biotechnology Co., Ltd. Cat#JL10484 Mouse IL-10 ELISA Kit Jianglai Biotechnology Co., Ltd. Cat#JL20242 Mouse lipocalin-2 (LCN2/NGAL) ELISA Kit Jianglai Biotechnology Co., Ltd. Cat#JL11556 High-sensitivity dsDNA quantification kit Yeasen Cat#12640ES60 Bright-Lumi Firefly Luciferase Reporter Gene Assay Kit Beyotime Cat#RG051S TUNEL assay kit Beyotime Cat#C1086 eBioscience Intracellular Fixation & Permeabilization Buffer Set Invitrogen Cat#88-8824-00 eBioscience Foxp3/Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 Ficoll-Paque density gradient medium TBDscience Cat#LTS1077-1 SteadyPure Universal RNA Extraction Kit AG Cat#21024 |